Two-photon holographic microscope system for 3D neuronal circuit imaging and stimulation
This technology is a fully integrated holographic microscope system capable of simultaneous, high-resolution imaging and precise stimulation of 3D neuronal circuits using optogenetics.
Unmet Need: Advanced and precise neuronal circuit imaging and stimulation
Current neuro-imaging techniques often lack the capability to simultaneously image and stimulate neuronal circuits with high precision and resolution, especially in three dimensions, in vivo. Many systems face limitations in speed, spatial resolution, and flexibility to simultaneously stimulate and record from select groups of neurons. This technological gap hinders the detailed study and manipulation of complex neuronal networks in vivo, which remains crucial for understanding brain function and disorders. The proposed technology addresses these shortcomings by offering a fast, high-resolution, and flexible platform for both imaging and stimulation of 3D neuronal circuits.
The Technology: Integrated holographic system for high-resolution neuronal imaging and stimulation
This holographic module for microscopy integrates advanced holography for simultaneous stimulation and imaging of neuronal cells, in vivo. It features software-controlled remote focusing, point spread function sculpting, hologram power optimization, and adaptive optics. These capabilities allow for precise manipulation and observation of neuronal circuits in three dimensions with single-cell accuracy. The technology merges recent developments in holography, providing a unified solution for detailed and dynamic studies of neural circuits.
Applications:
- Advanced imaging platform for neuronal cells and circuits
- Software-driven holography for neuroscience research
- Simultaneous optogenetic stimulation and visualization of neurons in vivo
- Remote focusing and adaptive optics in microscopic imaging
- Can be applied to both two-photon and single-photon microscopy for in vivo brain studies
Advantages:
- Simultaneous high-resolution imaging and stimulation of 3D volumes
- Software-controlled operation enabling precise targeting and manipulation
- Capable of single-cell precision in neuronal circuit studies
- Versatile for both academic research and potential clinical applications
- Software to use holography for:
- remote focusing
- PSF sculpting
- hologram power optimization
- adaptive optics
Lead Inventor:
Patent Information:
Patent Pending
Tech Ventures Reference:
IR CU23132
Licensing Contact: Kristin Neuman